Data Sheet

Background

Tubulin is a small family of globular proteins in nearly all eukaryotic cells. The most common members of this family are α-tubulin and β-tubulin, the proteins that make up microtubules. To form microtubules, the dimers of α- and β-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After a dimer is incorporated into microtubule, the molecule of GTP bound to the β-tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule filament. Thus, the binding of β-tubulin to GTP/GDP influences the formation of microtubules, and GTP cycle is essential for the dynamic instability of microtubule in cells.

Source

This is a mouse monoclonal antibody, which was raised against the full-length α-tubulin of human origin.

Gene Symbol

TUBA1A (Human)

Isotype

IgM

Physical Form

Freeze-dried powder from 1 × PBS solution

Specificity

This antibody detects the α-tubulin of human and mouse origins. Other species have not been tested yet.

Molecular Weight

50 kDa (α-tubulin), but it appears as ~55 kDa in SDS-PAGE.

Application

Western blotting (WB, dilution range: 1:1,000 - 10,000). Other applications have not been tested yet.

Storage

Store freeze-dried powder at 2 - 8°C upon arrival. When ready to use, rehydrate with 0.1 ml dH₂O and centrifuge if not clear. For long-term storage, make aliquots and keep them at -20°C or below. Avoid repeated freezing and thawing cycles.

Data

>> Western blotting: HT-1080 (A) and NIH/3T3 (B) cell extracts prepared in 1% Triton-X lysis buffer.

Important Note

This product is intended for research use only, not for use in human therapeutic or diagnostic procedures.