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Data Sheet

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GST (6F5) Antibody ()


Background

Glutathione S-transferase (GST) (EC 2.5.1.18) is an enzyme that catalyzes the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates to detoxify endogenous compounds. The GST encoded by parasitic worm Schistosoma japonicum, which has little homology to its mammalian counterpart, had been widely used as a fusion patner for expression and purification of foreign proteins in E. coli. GST-tag is easy to detect and it has little effect on the biological function of the protein of interest. Alternatively, a GST-tag can be removed by enzymatic cleavage on particular site(s), which was expressed from a well-designed plasmid vector.


Source

This is a mouse monoclonal antibody, which was raised against the full-length GST of S. japonicum origin.


Gene Symbol (ID)

GST (S. japonicum)


Isotype

IgG2a


Purity

Protein A-purified antibodies in 1 × PBS containing 0.02% sodium azide.


Specificity

The GST (6F5) antibody detects GST-tag alone and its fusion proteins.


Molecular Weight

26 kDa (GST)


Application

Western blotting (WB, dilution range: 1:500 - 5,000). Other applications have not been tested yet.


Storage

Keep at 2 - 8°C upon arrival (stable for six months from the date of shipment). For long-term storage, make aliquots and keep them at -20°C or below. Avoid repeated freeze-and-thaw cycles.


Data

>> Western blotting: E. coli bacterial lysate expressing recombinant S. japonicum GST.


Important Note

This product is intended for research use only, not for use in human therapeutic or diagnostic procedures.