RORγ (5D8) Antibody ()
The retinoid-related orphan receptors (RORs) consist of three members α, β and γ, which belong to the nuclear receptor superfamily and are implicated in the regulation of various metabolic pathways, energy homeostasis and some immunological processes. For example, RORα and RORβ are required for the maturation of photoreceptors in the retina; and RORγ is essential for the development of lymph nodes. Moreover, RORs are believed to have potential roles in several pathologies including osteoporosis, several autoimmune diseases, asthma, obesity and cancer. In human, there are two transcript variants of RORγ with different sizes: RORC1 is the longer transcript that encodes RORγ to be broadly expressed; and RORC2 is the shorter isoform that encodes RORγt, which is known to require during differentiation of Th17 cells. Both RORC1 and RORC2 share identical nucleotide sequences from exon 3 through the end.
This is a mouse monoclonal antibody raised against amino acids 2 – 13 of the RORγ of human origin.
Gene Symbol (ID)
Protein A-purified antibodies in 1 × PBS with 0.02% sodium azide.
This antibody detects the RORC1, but not RORC2 isoform of RORγ of human origin. Cross-reaction with other species has not been tested.
57.6 kDa (RORC1), but it appears as ~63 kDa in SDS-PAGE.
Western blotting (WB, dilution range: 1:500 - 5,000). Other applications have not been tested yet.
Keep at 2 - 8°C upon arrival (stable for six months from the date of shipment). For long-term storage, make aliquots and keep them at -20°C or below. Avoid repeated freeze-and-thaw cycles.
>> Western blotting: HT-1080 cell extracts prepared in 1% Triton-X lysis buffer.
This product is intended for research use only, not for use in human therapeutic or diagnostic procedures.